HomeOnline CataloguePCRPCR – DNA PolymerasesStandard PCR Taq DNA Polymerase

Taq DNA Polymerase

Top quality general application Taq DNA polymerase.

  • General & diagnostic PCR.
  • High purity.
  • No "proofreading".
  • Separate enzyme and 10x buffers.
  • Contains both, non-colored and colored 10x PCR buffers for direct gel loading

    Detailed Product Description
     English Version
     Deutsche Version

    Quantity Package Cat-No. Price in €
    200 uE2500-0126.50
    500 uE2500-0458.00
    1000 uE2500-0299.00
    5000 uE2500-03480.00
    200 u / Kit (+ dNTPs)EK2500-0133.50
    500 u / Kit (+ dNTPs)EK2500-0475.50
    1000 u / Kit (+ dNTPs)EK2500-02134.00
    5000 u / Kit (+ dNTPs)EK2500-03655.00

    Pure. Performant. No contaminating DNA.

    Figure 1: Taq DNA Polymerase - Package Contents
    • Taq DNA Polymerase
    • 10x Buffer Pol A (Optimization reaction buffer without MgCl2)
    • 10x Buffer Pol B (Standard reaction buffer containing MgCl2)
    • 10x Buffer Pol C (Purple coloured reaction buffer, for direct gel loading)
    • 25 mM MgCl2 solution
    Taq DNA Polymerase - Kit Package Contents
    • Same as Taq DNA Polymerase, plus ultrapure dNTP solution
    Application Example

    Figure 2: PCR amplification with Taq DNA Polymerase (Cat. No. E2500) in a size range between ~1 and 15 kb. Molecular weight markers MW1 (→ Perfect™ 1kb DNA Ladder), MW2 (Lambda-DNA [Phage λ, GenBank J02459] / → HindIII). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Taq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

    • All-Purpose Taq: For all PCR applications not requiring "HotStart". Great all-purpose DNA polymerase for general PCR applications.
    • High Quality: High purity and efficient enzymatic activity as a result of thorough but gentle enzyme purification.
    • Strict Quality Control: Optimized towards high enzyme quality: Taq DNA polymerase is continuously and extensively monitored.
      • No false positives: No detection of PCR-amplificable DNA, as tested in 40+ cycle PCR reactions with various amplification targets of human, eukaryotic, bacterial and viral origin (including bacterial 16S rRNA genes).
      • Extreme Purity: PAGE gel analysis of purified enzyme shows presence of just one single band, as one would expect for a >95% pure preparation.
      • Devoid of Nucleases and Proteases: Sensitive enzymatic tests reveal absence of any contaminating nucleases or proteases.
      • Strict quality controls ensure continuous reproducible high enzyme purity and performance, as opposed to certain infamous "crude extracts with intrinsic DNA polymerase activity" offered by some third parties.
      • Precise Conformity with Unit Specification: Strict and precise adjustment of enzymatic activity towards the regular, commonly accepted unit definition. One unit of enzyme is exactly one unit - and not less. At 74°C, one unit catalyzes the incorporation of precisely 10 nmoles of dNTP into acid-insoluble material within 30 min, no more, no less.
    • Excellent Price-Performance ratio: Good value for money.
    • Recombinant: Optimized, improved performance as compared to → Native Taq DNA Polymerase [Cat. No. E2504]).
    • All Buffers Inclusive: Ships complete with non-colored plus with colored buffers for direct gel loading.
    • Enzyme Properties: Intrinsic exonuclease activities: 5'-3' present, 3'-5' none. No proofreading activity.
    • Cloning: Enables both TA- and blunt end cloning.
    • Also available as: Taq PCR Master Mix [Cat. No. E2520]).
    Additional Resources

     PCR Logsheet

     How to trigger Reverse Transcriptase Activity of Taq DNA Polymerase (Contributed Protocol)