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Perpetual OptiTaq Master Mix

"Hot Start" OptiTaq DNA polymerase (Taq+Pfu), MAB inhibited.
  • Robust, long "HotStart" PCR.
  • Moderate "proofreading".
  • Convenient, stable master mix.
  • Contains separate, optional Color Load solution for direct gel loading.

Detailed product description
    English version       Deutsche Version  

Quantity Package Cat-No. Price in €
100 reactions
(100 x 50 μl)
200 reactions
(200 x 50 μl)
500 reactions
(500 x 50 μl)

Application Example

Figure 1: PCR amplification using EURx Perpetual OptiTaq DNA Polymerase.
A 2.5 kb PCR amplicon was amplified with Perpetual OptiTaq DNA Polymerase either in stand-alone or in master mix format, respectively.
  • Lane MW (1): molecular size marker- Perfect 1 kb DNA Ladder (Cat. No. E3130).
  • Lanes "Separate Components" (2,3): PCR amplification reactions using 1.25 U Perpetual OptiTaq DNA Polymerase, Pol Buffer B and dNTPs.
  • Lanes "Master Mix" (4,5): PCR amplification reactions using Perpetual OptiTaq PCR Master Mix (2x), after 25 freeze-thaw cycles.
  • Lanes "Master Mix + Dye" (6,7): PCR amplification reactions using Perpetual OptiTaq PCR Master Mix (2x) and 10 x Color Load, after 25 freeze-thaw cycles.
Result: Following 25 freeze-thaw cycles, OptiTaq Master Mix still displayed similar performance to OptiTaq DNA Polymerase with separately stored components and not subjected to repeated freeze-thawing.

Figure 2: Robust and sensitve: Short, medium and long range HotStart PCR amplifications with Perpetual OptiTaq DNA Polymerase (Cat. No. E2720) in a size range between ~1 and 20 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual OptiTaq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

Perpetual OptiTaq DNA Polymerase - Package Contents
  • Perpetual OptiTaq Master Mix (2x)
  • PCR Water
  • 10x Color Load (optional buffer, for direct gel loading)
Additional Resources

 PCR Master Mix Logsheet

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