HomeOnline CataloguePCRPCR – PCR Master Mixes"Hot Start", Non−"Proofreading" Perpetual Taq Master Mix

Perpetual Taq Master Mix

"Hot Start" Taq DNA polymerase, MAB inhibited.

  • Fluorescent PCR methods, genotyping, diagnostic PCR.
  • No "proofreading".
  • Convenient, stable master mix.
  • Contains separate, optional Color Load solution for direct gel loading.

Detailed Product Description
 English Version
 Deutsche Version

Quantity Package Cat-No. Price in €
100 reactions (50 μl)E2740-0175.00
200 reactions (50 μl)E2740-02145.00
500 reactions (50 μl)E2740-03290.00



Application Examples


Figure 1: Comparisonal HotStart PCR amplification of an 1.1 kb amplicon (human ccr5 gene) with Perpetual Taq DNA Polymerase (Cat. No. E2700, non-master-mix format)) and ready-to-use Perpetual Taq PCR Master Mix (Cat. No. 2740). Molecular weight marker MW (Perfect™ 1kb DNA Ladder). Each PCR reaction: 1.25 U Perpetual Taq DNA Polymerase, 0.2 mM dNTPs; 50 µl reaction volume. Lanes 2-3: EURx Perpetual Taq DNA Polymerase (non-master-mix format); 4-5: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles, to demonstrate tolerance for repeated freezing and thawing), 6-7: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles) plus optional Color Load (for direct post-PCR gel loading).



Figure 2: Sensitive HotStart PCR amplification with Perpetual Taq DNA Polymerase (Cat. No. E2700) in a size range between ~1 and 15 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual Taq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

Perpetual Taq PCR Master Mix (2x) - Package Contents
  • Perpetual Taq PCR Master Mix (2x)
  • PCR Water (Nuclease Free)
  • 10x Color Load (Optinal reaction component: Coloured reaction buffer, for direct gel loading)
    Optionally supplied, if needed:
  • 25 mM MgCl2 solution. If required, please request this component along with your order.
Additional Resources

 PCR Master Mix Logsheet

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