Figure: Short, medium and long distance high fidelity PCR amplification with PfuPlus! DNA Polymerase (Cat. No. E1118) in a size range between ~1 and 20 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions. For further reaction parameters see manual.
Color PfuPlus! DNA Polymerase - Package Contents
Color PfuPlus! DNA Polymerase
10x Color PfuPlus! Buffer
25 mM MgSO4 solution
Color PfuPlus! DNA Polymerase - Kit Package Contents
Same as PfuPlus! DNA Polymerase, plus ultrapure dNTP solution
Color PfuPlus! DNA Polymerase is supplemented with two tracing dyes (yellow and red). These dyes allow direct loading of PCR products to agarose gels. Additionally, these dyes provide an optical control for a complete mixing during reaction setup by visualizing the complete suspension of polymerase in the reaction mix. This may prove useful for less experienced users such as teaching classes and student courses. In this Color edition, these dyes are mixed directly to the polymerase . As a consequence, the Color enzyme edition does not allow the end user to run PCR reactions without addition of dyes.